Microarray technologies with antibodies, oligonucleotides, and nanoparticles

 

Multi-analyte immunoassays on microarrays and on multiplex DNA microarrays have been described for quantitative analysis of small organic molecules (e.g., antibiotics, drugs of abuse, small molecule toxins), proteins (e.g., antibodies or protein toxins), and microorganisms, viruses, and eukaryotic cells. In analytical chemistry, multi-analyte detection by use of analytical microarrays has become an innovative research topic because of the possibility of generating several sets of quantitative data for different analyte classes in a short time. Chemiluminescence (CL) microarrays are powerful tools for rapid multiplex analysis of complex matrices. A wide range of applications for CL microarrays is described in the literature dealing with analytical microarrays. The motivation for this presentation is to summarize the current state of CL-based analytical microarrays. Combining analysis of different compound classes on CL microarrays reduces analysis time, cost of reagents, and use of laboratory space. Applications are discussed, with examples from food safety, water safety, environmental monitoring, diagnostics, forensics, toxicology, and biosecurity. The potential and limitations of research on multiplex analysis by use of CL microarrays are discussed.

Reinhard Niessner

Prof. Dr. Reinhard Niessner is the Head of the Institute of Hydrochemistry and Chair of Analytical Chemistry at the Technical University of Munich, Germany. His research interests are within environmental analytical chemistry, especially devoted to applications of laser spectroscopy, nanoparticle characterization, and microarray technologies. The main subject of interest are aerosols, hydrocolloids and biofilms, within hydro- and atmosphere. He received several prestigious awards, and is currently author or coauthor of 594 publications.