ANALYSIS OF DIOXINS ACCORDING TO COMMISSION REGULATIONS (EU) 589/2014 AND (EU) 709/2014 BY GC TRIPLE QUADRUPOLE MASS SPECTROMETRY

 

Already since 2012 it has been allowed to perform screening and quantitative measurements for dioxin analysis on low resolution mass spectrometers. In 2014 two new European Regulations for Food and Feed have been set into action allowing also confirmation of positive findings by TQ Mass Spectromety. 12  instead of high resolution mass spectrometry if you can show the compliance with some analytical criteria since 2012 already.
The following criteria most be fulfilled by the method:
-    Each group requires at least one 13C-labelled homologue per group of tetra- to octachlorinated PCDD/PCDF.
-    Recovery of internal standards has to be between 30% and 140% for screening methods.
-    Separation of the isomers 1,2,3,4,7,8 and 1,2,3,6,7,8-HxCDF has to be sufficient (<25% overlay peak to peak).
-    The calibration curve has to cover the relevant concentrations starting from the level of detection.
Additional requirements which have been laid down with Regulations EU 589 and 709/2014 method requirements for confirmation of dioxins, and related compounds are
-    Unit resolution for both analytical Quadrupoles
-    Ion ratio tolerance <15%
-    At least two significant precursor with one significant product ion each
Based on data from reference material in different matrices we are going to show, how modern high precision Triple Quadrupole mass spectrometers perform in these tasks. The compliance with all requirements is going to be shown on samples from food, feed and environmental with certified reference material provided by the Institute for Reference Materials and Measurements (IRMM). Pitfalls and possibilities are covered in this overview.

Gordon van 't Slot



Gordon van 't Slot is working as Senior Application Chemist at Bruker Daltonik GmbH in Bremen. His responsibility are the Triple Quadrupole Mass Spectrometers either coupled to GC- or LC-systems. Beside developing Applications, he gives training courses for these products.
Gordon van 't Slot studied Food Chemistry in Muenster, Germany and finished studies in 2005 and holds his State Certification since 2006. Afterwards he received his PhD from the University of Muenster in the working group of Prof. Dr. Hans-Ulrich Humpf.
Before joining Bruker Daltonik GmbH Gordon van 't Slot worked as a lab manager in a pesticide residue lab.
His major application interests are food and environmental with special focus on POPs and metabolism of xenobiotics in mammals.

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